Compensation in flowjo series#
In addition, we invite outside speakers to the Department of Immunology and Microbiology seminar series to discuss advanced technology. Special applications (intracellular cytokines, cell proliferation, apoptosis, phosphorylation assays, etc) are offered as lecture/wet laboratory formats in a Flow Cytometry Symposium each year. At following sessions, one-on-one supervision is provided for as long as required, with the eventual goal of making the scientist, student, or technician a careful and independent user of the facility.Ī critical component to this training is the effective use of written, up-to-date standard operating procedures (SOPs), which are available on this website as well as at each instrument. Subsequent training sessions involve a user’s own samples – with training specific to the needs of that user. Initial training at the flow cytometry instrument involves standard samples.
Compensation in flowjo software#
Instrument training is available for individual researchers by an experienced staff member and covers basic operational procedures including acquisition software and Shared Resource policies. Designing antibody cocktails for multi-color flow cytometry.Fluorochromes, lasers, filters, and PMTs.We have also introduced Omniviz (BioWisdom Ltd, Cambridge, UK) software to simplify analysis of mechanistic studies that have multiple patients, each with multiple time points. We are beginning to phase in GemStone (Verity Software House, Topsham, ME) software, a new paradigm for analyzing high-dimensional, flow cytometry data based on probability state modeling. The Shared Resource staff is on hand to field questions. Flow cytometry software includes Cell Quest, FlowJo, Modfit, and Winlist. There are 5 work-stations, including 3 G4 MacPro's, a 27" iMAC and a G5 PowerPC with dual 27" monitors. We have a room in the Flow Cytometry Shared Resource that is dedicated to investigator-performed data analysis. These lectures are posted on our website, together with self-help modules.Įrrors in sample acquisition and/or data analysis are common among poorly trained users and can lead to misleading and/or unreliable data. This lecture series occurs approximately three times per year. Weekly formal lectures ( seminar schedule) cover the basic concepts of flow cytometry, including compensation, fluorochrome selection for multi-color assays, sample preparation, software for data analysis, together with the principles of each immunoassay that is available for use. All users receive basic education on the science and technology of flow cytometry, cell sorting, and immunoassays through both formal and informal programs. The Shared Resource staff is committed to providing training and education for all the facility users.
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